Protein

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Genbank accession

XUU32662.1 [GenBank]

Protein name

tail spike protein

RBP type

Evidence Phold

Probability 1,00

TSP

Evidence DepoScope

Probability 1,00

TSP

Evidence RBPdetect

Probability 0,90

TSP

Evidence RBPdetect2

Probability 0,95

Protein sequence

MSTITQFPSGNTQYRIEFDYLARTFIVVTLVNSSNPTLNRVLEVGRDYRFLNPTMIEMLVDQSGFDIVRIHRQTGTDLVVDFRNGSVLTASDLTNSELQAIHIAEEGRDQTVDLAKEYADAAGSSAGNAKDSEDEARRIAESIREAGLIGYITRRSFEKGYNVTTWSEVLLWEEDGDYYRWDGTLPKNVPAGSTPETSGGIGLGAWVSVGDAALRSQISNPEGAILYPELHRARWLDEKDVRGWGAKGDGVTDDTAALTSALNDIPVGQKINGNGKTYKVTSLPDISRFINTRFVYERIPGQPLYYASEEFVQGELFKITDTPYYNAWPQDKAFVYENVIYAPYMGSDRHGVSRLHVSWVKSGDDGQTWSTPEWLTDLHPDYPTVNYHCMSMGVCRNRLFAMIETRTLAKNELTNCALWDCPMSRSLHLTGGITKAANQRYATIHVPDHGLFVGDFVNFSNSSVTGVSGDMTVATVIDKDNFTVLTPNQQTSDSNNAGKNWHMGTSFHKSPWRKTDLGLIPRVTEVHSFATIDNNGFVMGYHQGDVAPREVGLFYFPDAFNSPSSYVRRQIPSEYEPDASEPCIKYYDGVLYLITRGTRGDRLGSSLHRSRDIGQTWESLRFPHNVHHTTLPFAKVGDDLIMFGSERAENEWEAGAPDDRYKASYPRTFYARLNVNNWNADDIEWVNITDQIYQGGIVNSGVGVGSVVVKDNYIYYIFGGEDHFNPWTYGDNSAKDPFKSDGHPSDLYCYKMKIGQSNHVSRDFRYGAVPNRAVPVFFDTNGIRTVPAPMEFTGDLGLGHVTLRASTSSNIRSEVLMEGEYGFIGKSIPTDNPAGQRIIFCGVKAPVQRLAHRLRFMVQITPTQGE

Physico‐chemical properties

protein length:	866 AA
molecular weight:	96811,72160 Da
isoelectric point:	5,46151
aromaticity:	0,10970
hydropathy:	-0,43984

Domains

Domains [InterPro]

DC_1609
STR
1–136

DC_1609 G3DSA:2.10.10.80 G3DSA:3.30.750.60 IPR024428 IPR044914

IPR005604
ATT
10–113

IPR005604 IPR040775 IPR036278 SSF69349

DC_1280
ATT
105–287

DC_1280 IPR024428 IPR024430

IPR024429
ENZ
246–312

IPR024429 IPR001724 IPR001724 IPR023366 IPR001724 IPR001724 IPR001724 IPR044914

G3DSA:2.120.10.10
STR
314–754

G3DSA:2.120.10.10 IPR001724

IPR024427
ENZ
424–506

IPR024427

IPR001724
Unmapped
498–521

IPR001724

XUU32662.1

1 866

Architecture

ATT

STR

ATT 1-312 | STR 313-851 |

Legend: ATT STR RBD CBM LEC ENZ CHP LNK TAS TTP UNK Unmapped

Domains [InterPro]

Domain ID	Category	Cluster	Start	End	Layer	Name	Pref Zone	Confidence	Evidence	Support
DC_1609	STR	DC_1609	1	136	Novel HMM	DC_1609	Central	Low	Positional only	263 proteins / 263 hits
IPR005604	ATT	DC_0240	10	113	InterPro cluster	Bacteriophage T7 tail fibre protein-like, N-terminal domain	Central	Medium	Text match	1326 proteins / 1328 hits
DC_1280	ATT	DC_1280	105	287	Novel HMM	DC_1280	N-terminal	Low	Positional only	90 proteins / 90 hits
G3DSA:2.10.10.80	ATT	G3DSA:2.10.10.80	150	220	Merged direct domain	G3DSA:2.10.10.80	N-terminal	Low	Positional only	383 proteins / 383 hits
IPR040775	RBD	DC_0821	156	211	InterPro cluster	Tail spike TSP1/Gp66, N-terminal domain	Central	High	Text match	1281 proteins / 1285 hits
G3DSA:3.30.750.60	ATT	G3DSA:3.30.750.60	241	312	Merged direct domain	G3DSA:3.30.750.60	N-terminal	Low	Positional only	2 proteins / 2 hits
IPR036278	STR	IPR036278	245	760	Merged direct domain	Sialidase superfamily	Central	Low	Positional only	56 proteins / 56 hits
IPR024429	ENZ	DC_0802	246	312	InterPro cluster	Endosialidase, N-terminal extension domain	N-terminal	High	Text match	84 proteins / 84 hits
IPR024428	ENZ	DC_0114	313	755	InterPro cluster	Endosialidase, beta-propeller domain	Central	High	Text match	92 proteins / 92 hits
IPR024428	ENZ	DC_0114	313	754	InterPro cluster	Endosialidase, beta-propeller domain	Central	High	Text match	92 proteins / 92 hits
IPR001724	Unmapped	-	314	338	InterPro	Glycosyl hydrolase 58	-	-	-	-
G3DSA:2.120.10.10	STR	G3DSA:2.120.10.10	314	754	Merged direct domain	G3DSA:2.120.10.10	Central	Low	Positional only	42 proteins / 42 hits
IPR001724	Unmapped	-	379	407	InterPro	Glycosyl hydrolase 58	-	-	-	-
IPR023366	STR	IPR023366	424	510	Merged direct domain	ATP synthase subunit alpha, N-terminal domain-like superfamily	Central	Low	Positional only	44 proteins / 44 hits
IPR024427	ENZ	DC_0382	424	506	InterPro cluster	Endosialidase, beta-barrel domain	Central	High	Text match	86 proteins / 86 hits
IPR001724	Unmapped	-	498	521	InterPro	Glycosyl hydrolase 58	-	-	-	-
IPR001724	Unmapped	-	568	594	InterPro	Glycosyl hydrolase 58	-	-	-	-
IPR001724	Unmapped	-	646	672	InterPro	Glycosyl hydrolase 58	-	-	-	-
IPR001724	Unmapped	-	702	726	InterPro	Glycosyl hydrolase 58	-	-	-	-
IPR044914	RBD	IPR044914	755	851	Merged direct domain	Endosialidase, C-terminal domain superfamily	C-terminal	Low	Positional only	39 proteins / 39 hits
IPR024430	ENZ	DC_0521	757	843	InterPro cluster	Endosialidase, C-terminal domain	C-terminal	High	Text match	95 proteins / 95 hits
IPR044914	RBD	IPR044914	757	851	Merged direct domain	Endosialidase, C-terminal domain superfamily	C-terminal	Low	Positional only	39 proteins / 39 hits
SSF69349	STR	SSF69349	761	843	Merged direct domain	SSF69349	Central	Low	Positional only	153 proteins / 153 hits
IPR001724	Unmapped	-	803	829	InterPro	Glycosyl hydrolase 58	-	-	-	-

Tail Spike Domain Segmentation

This protein has been segmented into three structural domains: N-terminal, central domain, and C-terminal.

Domain Layout

N-terminal

Central

C-terminal

XUU32662.1

1 866

Domain	Start	End	Length (AA)	Confidence
N-terminal	1	255	255	0,9975
Central domain	256	577	323	0,8366
C-terminal	578	866	288	0,4690

Legend: N-terminal Central domain C-terminal

3D Structure with Domain Coloring

The structure is colored according to the domain segmentation: N-terminal (blue), Central (green), C-terminal (pink).

Domain Coloring

N-terminal
1-255

Central
256-577

C-terminal
578-866

Taxonomy

	Name	Taxonomy ID	Lineage
Phage	Escherichia phage vB_Eco_1RS [NCBI]	3423639	Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes
Host	Escherichia coli [NCBI]	562	cellular organisms > Bacteria > Pseudomonadati > Pseudomonadota > Gammaproteobacteria > Enterobacterales

Coding sequence (CDS)

Genbank protein accession

XUU32662.1 [NCBI]

Genbank nucleotide accession

PV615263.1 [NCBI]

CDS location

range 31564 -> 34164
strand -

CDS

ATGTCCACGATTACACAATTCCCTTCAGGAAACACTCAGTACAGGATTGAGTTCGACTACCTAGCCAGAACGTTTATTGTTGTTACGCTGGTGAATAGCTCTAACCCTACCCTGAACCGTGTACTGGAAGTTGGTCGAGATTATCGATTCCTTAATCCAACGATGATTGAGATGTTGGTTGACCAATCAGGTTTCGACATCGTTCGTATTCACCGTCAGACTGGAACTGACTTAGTGGTAGACTTCAGGAATGGCTCGGTGTTGACGGCTAGTGACCTGACCAATTCAGAGCTTCAGGCTATCCATATTGCAGAAGAAGGTCGAGACCAAACGGTTGACTTAGCGAAGGAATATGCCGATGCTGCTGGTAGCTCTGCTGGCAACGCTAAGGATAGCGAGGACGAAGCACGACGAATCGCTGAGAGTATCAGGGAAGCTGGTCTAATTGGCTATATCACCCGTCGCTCCTTCGAGAAAGGCTACAACGTTACAACATGGAGCGAGGTCCTGCTATGGGAAGAGGATGGTGATTATTACCGCTGGGATGGTACGCTTCCAAAGAACGTTCCTGCTGGTTCAACTCCTGAAACTTCCGGTGGGATTGGATTAGGTGCGTGGGTTAGTGTTGGTGATGCTGCTTTAAGAAGTCAGATTTCAAATCCTGAAGGGGCAATACTCTACCCTGAATTACATAGAGCACGCTGGCTTGATGAAAAAGATGTTCGTGGTTGGGGTGCTAAAGGGGATGGTGTCACTGATGATACAGCAGCGTTAACGAGCGCATTAAACGACATACCGGTTGGACAAAAGATTAATGGCAATGGGAAGACATATAAGGTCACATCCCTGCCTGATATTAGTCGCTTCATCAACACTCGTTTCGTTTATGAGCGCATTCCCGGACAACCTCTTTATTATGCCTCTGAAGAATTTGTTCAGGGTGAGTTGTTCAAAATAACGGACACCCCTTATTACAATGCGTGGCCTCAGGATAAAGCGTTCGTATATGAGAATGTGATATATGCACCTTACATGGGTAGTGATCGTCATGGCGTTAGTCGTCTGCATGTATCATGGGTTAAGTCTGGTGATGATGGTCAAACATGGTCTACTCCAGAGTGGTTAACTGATCTGCATCCAGATTACCCTACAGTGAACTATCATTGTATGAGTATGGGTGTATGTCGCAACCGTCTGTTTGCCATGATTGAAACACGTACTTTAGCCAAGAACGAATTAACCAATTGTGCATTGTGGGATTGCCCCATGTCTCGTAGCCTGCATCTTACTGGTGGTATCACTAAAGCTGCAAATCAGCGATATGCAACAATACATGTACCAGACCACGGACTATTCGTGGGTGATTTTGTTAACTTCTCTAATTCTTCGGTAACAGGTGTATCCGGTGATATGACTGTTGCAACCGTAATAGATAAGGACAACTTCACGGTTCTTACACCCAACCAGCAGACTTCAGATTCGAATAACGCTGGAAAGAATTGGCACATGGGTACTTCTTTCCATAAGTCTCCATGGCGTAAGACAGACCTTGGTCTAATCCCTCGTGTCACAGAGGTGCATAGCTTTGCTACTATTGATAACAACGGCTTTGTTATGGGCTATCATCAAGGCGATGTAGCTCCACGCGAAGTTGGGCTTTTCTACTTCCCTGATGCTTTCAATAGCCCATCTAGTTATGTTCGTCGTCAGATACCATCTGAGTATGAACCAGATGCGTCAGAGCCGTGCATCAAGTACTATGACGGTGTATTATACCTTATCACTCGTGGCACTCGTGGGGATAGACTAGGTAGCTCTTTGCATCGTAGTAGAGACATAGGTCAGACTTGGGAGTCACTGAGATTTCCACATAATGTTCATCATACTACATTACCTTTTGCTAAAGTAGGGGATGACCTTATTATGTTTGGTTCAGAACGTGCAGAAAATGAATGGGAAGCAGGTGCACCAGATGATCGTTACAAGGCATCTTACCCTCGCACCTTCTATGCACGCTTGAATGTAAACAATTGGAATGCAGATGATATTGAATGGGTTAACATCACTGACCAGATTTATCAGGGCGGAATAGTTAACTCAGGTGTAGGTGTTGGTTCGGTAGTTGTGAAGGATAATTACATCTACTACATATTTGGTGGAGAGGACCATTTTAACCCATGGACATATGGAGATAACTCAGCGAAGGATCCCTTCAAATCGGATGGTCATCCCTCAGATTTGTATTGCTACAAAATGAAGATTGGCCAAAGCAATCATGTTTCCCGTGACTTCAGGTATGGTGCTGTACCTAACAGAGCTGTTCCCGTGTTTTTTGATACGAATGGGATTCGCACTGTTCCTGCGCCAATGGAATTTACAGGGGATTTGGGTTTAGGTCATGTGACCCTTAGAGCCAGCACCAGTAGTAACATTCGCTCTGAAGTTTTAATGGAAGGTGAATATGGGTTTATCGGGAAGTCTATACCGACTGATAACCCGGCAGGGCAGCGCATCATATTTTGCGGGGTGAAGGCACCAGTTCAACGACTGGCGCACAGATTACGCTTTATGGTGCAAATAACACCGACTCAAGGCGAATAG

Genome Context

Tertiary structure

PDB ID

563eb1cf32b5ea2056f57fb668d4b979be8451501aae5537c1532c9f72ad9c3b

ColabFold

Source ColabFold

Method ColabFold

Resolution 0,7606

Oligomeric State monomer

Download PDB

Model Confidence

Very high
pLDDT > 90

High
90 > pLDDT > 70

Low
70 > pLDDT > 50

Very low
pLDDT < 50

Protein

Domains

Domains [InterPro]

Domains [InterPro]

Tail Spike Domain Segmentation

Tail Spike Domain Segmentation

3D Structure with Domain Coloring

Domain Coloring

Taxonomy

Coding sequence (CDS)

Coding sequence (CDS)

Genome Context

Genome Context

Tertiary structure

Predicted Aligned Error (PAE)

Model Confidence